Transformation of e coli cells with plasmid pamp

The teacher s need to prepare materials several days before the actual lab. Preparing the media for the transformation part of the lab will take longer than the regular transformation.

Transformation of e coli cells with plasmid pamp

Neurophysiology Virtual Lab pilot Neurophysiology is the study of nervous system function. Primarily, it is connected with neurobiology, psychology, neurology, clinical neurophysiology, electrophysiology, biophysical neurophysiology, ethology, neuroanatomy, cognitive science and other brain sciences.

Neuron Simulation Virtual Lab pilot This lab uses a graphical web-based Neuron simulator and models a section of excitable neuronal membrane using the Hodgkin-Huxley equations. Various experiments will deal with the several parameters of Hodgkin-Huxley equations and will model resting and action potentials, voltage and current clamp, pharmacological effects of drugs that block specific channels etc.

This lab complements some of the exercises in the Virtual Neurophysiology lab. Biochemistry Virtual Lab I Biochemistry is the study of the chemical processes in living organisms. It deals with the structures and functions of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules.

DNA Cloning with Plasmid Vectors - Molecular Cell Biology - NCBI Bookshelf

The experiments included in Biochemistry Virtual Lab I are fundamental in nature, dealing with the identification and classification of various carbohydrates, acid-base titrations of amino acids, isolation of proteins from their natural sources, etc.

Biochemistry Virtual Lab II Biochemistry Virtual Lab II deals with topics like enzymology, purification of plant pigments and natural products as well as estimation of iodine value and saponification value of fats and oils.

Population ecology Virtual Lab I A population is a collection of individuals of the same species that live together in a region. Population ecology is the study of populations especially population abundance and how they change over time. Crucial to this study are the various interactions between a population and its resources.

Studies on simple models of interacting species is the main focus this simulation oriented lab. Population ecology Virtual Lab II Population ecology is the study of populations especially population abundance and how they change over time. Studies based on models of predation, competition as seen in interacting species is the main focus this simulation oriented lab.

Lab II focuses on applied principles of population ecology for PG students. Immunology Virtual Lab I The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.

Immunology Virtual Lab II The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from no self, and laboratory techniques involving the interaction of antigens with specific antibodies.

Microbiology Virtual Lab I The study of microorganisms, which are unicellular or cell-cluster microscopic organisms. This includes eukaryotes such as fungi and, protists and prokaryotes. Viruses, though not strictly classed as living organisms, are also studied. Microbiology Virtual Lab II To study the biochemical properties of microorganisms, the various techniques employed in cultivation of fungi and viruses along with the molecular level analysis of microbial genome.

This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interactions between DNA, RNA and protein biosynthesis as well as learning how these interactions are regulated.

Cell biology Virtual Lab I Cell biology is an exciting and dynamic area that helps discover the fascinating world of cells.

It includes the study of the structure and organization, growth, regulation, movements and interaction of the cells. Cell biology is closely related to other areas of biology such as genetics, molecular biology, and biochemistry.

Cell biology Virtual Lab II Cell biology is an exciting and dynamic area that helps discover the fascinating world of cells.

Cell Biology

Bioinformatics Virtual Lab I Bioinformatics is a field which using techniques of informatics to gather, store, analyse and integrate biological data. This virtual lab is an introductory course for undergraduate students and deals with the storage and retrieval of data from different biological databases like Gene, Pubmed, GEO, TAIR, Prosite etc.

Transformation of e coli cells with plasmid pamp

Bioinformatics Virtual Lab II This virtual laboratory is for undergraduate and postgraduate students to get a deeper understanding on the analysis of sequence data, its alignment and the evolutionary relationship.

The exercises mainly deal with the different algorithms in sequence alignment and provides a computational exploration to the use of various tools used for sequence alignment. This lab is targeted towards PG students with exercises that will allow one to learn visualising proteins in 3D, how to calculate distance among atoms, find active sites in protein structures and also delve into some structural analysis methods including docking and homology modeling.

Combining labs 1, 2 and 3 will give an overall understanding of commonly used computational methods in bioinformatics. Ecology Virtual Lab Ecosystems are a complex and delicate balancing game. Ecosystems have an extremely complex web of cause and effect. The addition or removal of one species affects many other species with which it might compete for,or provide food.

Bio-inspired Robotics Virtual Labs Remote Trigger This remote-triggerable online laboratory will teach experiments and offer to introduce biorobotics and neuronal robot techniques.

The focus is on practical skills in using simple electronics to reinforce application of bio-inspired ideas. Many experiments will help working towards thesis projects.

Virtual Biophysics Lab Remote Trigger This lab will provide an online experience via remote equipment to study biophysics and biophysical techniques.Rapid Colony Transformation of E. coli Cells with Plasmid DNA pAMP more by Danielle Diamante Antibiotic resistance is the ability of bacteria to resist the effects of an antibiotic, reducing the effectiveness of the treatment.

the Transformation Lab Experiment UsingE. coli and pFluoroGreen vector containing the pFluoroGreen plasmid into E. coli Plan an investigation individually and collaboratively and in it into a new cell. In transformation, competent cells take up “naked” DNA in the medium.

Not all cells. Regardless of which method of tranformation is used, outgrowth of bacteria following transformation allows repair of the bacterial surface and selection of recombinant cells if the newly acquired DNA conveys antibiotic resistance to the transformed cells.

In our experiment, E. coli +pAMP will transfer the ampicillin resistance gene to E.

[BINGSNIPMIX-3

coil -pAMP, thus transforming the E. coli cells. In our experiment, the plasmid pAMP is a circular DNA molecule that contains a BamHI and HindIII restriction site.

Transformation of Bacteria with GFP Figure 4. Expression of GFP: This is a diagram of what is occurring inside a bacterium transformed with the pGLO plasmid. The bacterium is plated on agar medium containing ampicillin and arabinose. To grow, the bacterium must contain a pGLO plasmid and be expressing the ampicillin resistance.

PROTEIN PRODUCTION USING TRANSFORMED ESCHERICHIA COLI 3 under UV light. The entire volume of liquid was allowed to drain through. Tube Product1 was checked for fluorescence.

Transformation of E. coli with Antibiotic Resistance